Virus:Article Title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Lab Cat# GFP-1020, RRID: AB_10000240 Rat anti-RFP (it recognizes also mCherry) Chromotek Cat# 5f8-100, RRID: AB_2336064 Rabbit anti-fibronectin Sigma-Aldrich Cat# F3648, RRID: AB_476976 Rabbit anti-Phospho-FAK (Tyr397) Thermo Fisher Scientific Cat# 44-624G, RRID: AB_2533701 Rabbit anti-Phospho-Smad1/5 (Ser463/465) (41D10) Cell Signaling Technology Cat# 9516, RRID: AB_491015 Mouse anti-Pan-Cadherin antibody clone CH-19 Sigma-Aldrich Cat# SAB4200731, RRID: AB_2904558 Goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11039, RRID: AB_2534096 Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 555 Thermo Fisher Scientific Cat# A-21434, RRID: AB_2535855 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Thermo Fisher Scientific Cat# A-21244, RRID: AB_2535812 CyTM3 AffiniPureTM F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) Jackson ImmunoResearch Labs Cat# 715-166-151, RRID: AB_2340817 Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Thermo Fisher Scientific Cat# C404010 NEB 5-alpha Competent E. coli New England Biolabs Cat# C2987 Chemicals, peptides, and recombinant proteins Dextran, Alexa FluorTM 647 10.000 MW Thermo Fisher Scientific Cat# D22914 Dextran Cascade BlueTM 10.000 MW Thermo Fisher Scientific Cat# D1976 D-Mannitol Sigma-Aldrich Cat# M4125 Paraformaldehyde 16% Solution (methanol-free) Agar Scientific Cat# AGR1026 Tissue-Tek O.C.T. .. Compound Sakura Finetek Cat# 4583 Sucrose Sigma-Aldrich Cat# S9378 DAPI (40,6-diamidino-2-phenylindole, dihydrochloride) Invitrogen Cat# D1306 Critical commercial assays RNeasy Plus Micro Kit QIAGEN Cat# 74034 Deposited data Animal vs. lateral ectoderm differentially-expressed genes This paper GSE251904 CHX-treated bmp7 mutants vs. CHX-treated bmp7 mutants injected with BMP2/7 protein differentially-expressed genes Greenfeld et al.37 GSE163047 Experimental models: Organisms/strains Zebrafish: AB wild-type MPI-CBG Dresden N/A Zebrafish: ABxTL wild-type MPI-CBG Dresden N/A Zebrafish: tg(sebox::eGFP) Ruprecht et al.20 ZDB-FISH-150901-6184 Zebrafish: tg(sebox::eGFP); tg(actb2::H2A-mCherry) This paper N/A Zebrafish: tg(actb2::H2A-mCherry) Krens et al.62 ZDB-FISH-150901-29861 Zebrafish: tg(sox17::eGFP) Chung et al.21 ZDB-FISH-150901-15761 Zebrafish: tg(actb2::mKate2-tpm3) This paper N/A (Continued on next page) Cell Reports 44, 115387, March 25, 2025 17
Article Title: Recruitment of Adult Precursor Cells Underlies Limited Repair of the Infected Larval Midgut in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-pH3 Millipore Cat# 06-570, RRID:AB_310177 rabbit anti-b-Galactosidase MP Biomedicals Cat# 0855976, RRID:AB_2334934 goat anti-b-Galactosidase MP Biomedicals Cat#0856028 mouse anti-Prospero DHSB Cat# Prospero (MR1A), RRID: AB_528440 mouse anti-Delta DSHB Cat# c594.9b, RRID:AB_528194 donkey anti-rabbit-555 Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543 donkey anti-goat-555 Thermo Fisher Scientific Cat# A-21432, RRID:AB_2535853 donkey anti-mouse-488 Thermo Fisher Scientific Cat# A-21202, RRID:AB_141607 donkey anti-mouse-647 Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542 Rabbit anti Phospho-Smad1/5 (Ser463/465) (41D10) Cell signaling Technology Cat# 9516, RRID: AB_491015 Chicken anti GFP Invitrogen Cat# A10262, RRID: AB_2534023 Rabbit anti RFP Invitrogen Cat# R10367 Goat Anti-Chicken IgG (H+L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A A11039, RRID: AB_142924 Bacterial and Virus Strains Erwinia carotovora ssp. carotovora 15 Basset et al., 2003 N/A Erwinia carotovora ssp. carotovora 15 pOM evf Basset et al., 2003 N/A Chemicals, Peptides, and Recombinant Proteins FD&C Blue #1 dye Spectrum Chemical FD110 Critical Commercial Assays QuantSeq 30 mRNA-Seq Library Prep Kit Lexogen SKU: 015.96 Deposited Data RNA-seq of adult and larval midguts with and without Ecc15 infection NCBI BioProject PRJNA553080 Experimental Models: Organisms/Strains Dmel/Canton-S (Cs) Bloomington RRID: BDSC_64349 Dmel/Su(H)TS (w; Su(H)-Gal4, UAS-CD8-GFP/CyO; tubGal80TS/TM6C) Zeng et al., 2010 N/A Dmel/Su(H)-lacZ (Su(H)GBE-lacZ) Furriols and Bray, 2001 N/A Dmel/Myo-lacZ (Yw; pLacW Myo-lacZ/CyO) Jiang et al., 2009 N/A Dmel/MyoTS (w; Myo1A-Gal4, UAS-GFP, Tub-Gal80TS) Jiang et al., 2009 N/A Dmel/TkvRNAi (y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS04510}attP40) Bloomington RRID: BDSC_57309 Dmel/UAS-Upd3 (w;UAS-Upd3/CyO) Buchon et al., 2009a N/A Dmel/UAS-lat;UAS-DomeDN (UAS-Latran; UAS-domeDCYT3-2) Makki et al., 2010 N/A Dmel/upd3-lacZ (Upd3.1-lacZ) Jiang et al., 2011 N/A Dmel/UAS-RasV12 (w[1118]; P{w[+mC] = UAS-Ras85D.V12}TL1) Bloomington RRID: BDSC_4847 Dmel/UAS-dpp (w[*]; P{w[+mC] = UAS-dpp.S}42B.4) Bloomington RRID: BDSC_1486 (Continued on next page) Cell Host & Microbe 26, 1–14.e1–e5, September 11, 2019 e1 ..
Recombinant:Article Title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Lab Cat# GFP-1020, RRID: AB_10000240 Rat anti-RFP (it recognizes also mCherry) Chromotek Cat# 5f8-100, RRID: AB_2336064 Rabbit anti-fibronectin Sigma-Aldrich Cat# F3648, RRID: AB_476976 Rabbit anti-Phospho-FAK (Tyr397) Thermo Fisher Scientific Cat# 44-624G, RRID: AB_2533701 Rabbit anti-Phospho-Smad1/5 (Ser463/465) (41D10) Cell Signaling Technology Cat# 9516, RRID: AB_491015 Mouse anti-Pan-Cadherin antibody clone CH-19 Sigma-Aldrich Cat# SAB4200731, RRID: AB_2904558 Goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11039, RRID: AB_2534096 Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 555 Thermo Fisher Scientific Cat# A-21434, RRID: AB_2535855 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Thermo Fisher Scientific Cat# A-21244, RRID: AB_2535812 CyTM3 AffiniPureTM F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) Jackson ImmunoResearch Labs Cat# 715-166-151, RRID: AB_2340817 Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Thermo Fisher Scientific Cat# C404010 NEB 5-alpha Competent E. coli New England Biolabs Cat# C2987 Chemicals, peptides, and recombinant proteins Dextran, Alexa FluorTM 647 10.000 MW Thermo Fisher Scientific Cat# D22914 Dextran Cascade BlueTM 10.000 MW Thermo Fisher Scientific Cat# D1976 D-Mannitol Sigma-Aldrich Cat# M4125 Paraformaldehyde 16% Solution (methanol-free) Agar Scientific Cat# AGR1026 Tissue-Tek O.C.T. .. Compound Sakura Finetek Cat# 4583 Sucrose Sigma-Aldrich Cat# S9378 DAPI (40,6-diamidino-2-phenylindole, dihydrochloride) Invitrogen Cat# D1306 Critical commercial assays RNeasy Plus Micro Kit QIAGEN Cat# 74034 Deposited data Animal vs. lateral ectoderm differentially-expressed genes This paper GSE251904 CHX-treated bmp7 mutants vs. CHX-treated bmp7 mutants injected with BMP2/7 protein differentially-expressed genes Greenfeld et al.37 GSE163047 Experimental models: Organisms/strains Zebrafish: AB wild-type MPI-CBG Dresden N/A Zebrafish: ABxTL wild-type MPI-CBG Dresden N/A Zebrafish: tg(sebox::eGFP) Ruprecht et al.20 ZDB-FISH-150901-6184 Zebrafish: tg(sebox::eGFP); tg(actb2::H2A-mCherry) This paper N/A Zebrafish: tg(actb2::H2A-mCherry) Krens et al.62 ZDB-FISH-150901-29861 Zebrafish: tg(sox17::eGFP) Chung et al.21 ZDB-FISH-150901-15761 Zebrafish: tg(actb2::mKate2-tpm3) This paper N/A (Continued on next page) Cell Reports 44, 115387, March 25, 2025 17
Article Title: Recruitment of Adult Precursor Cells Underlies Limited Repair of the Infected Larval Midgut in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-pH3 Millipore Cat# 06-570, RRID:AB_310177 rabbit anti-b-Galactosidase MP Biomedicals Cat# 0855976, RRID:AB_2334934 goat anti-b-Galactosidase MP Biomedicals Cat#0856028 mouse anti-Prospero DHSB Cat# Prospero (MR1A), RRID: AB_528440 mouse anti-Delta DSHB Cat# c594.9b, RRID:AB_528194 donkey anti-rabbit-555 Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543 donkey anti-goat-555 Thermo Fisher Scientific Cat# A-21432, RRID:AB_2535853 donkey anti-mouse-488 Thermo Fisher Scientific Cat# A-21202, RRID:AB_141607 donkey anti-mouse-647 Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542 Rabbit anti Phospho-Smad1/5 (Ser463/465) (41D10) Cell signaling Technology Cat# 9516, RRID: AB_491015 Chicken anti GFP Invitrogen Cat# A10262, RRID: AB_2534023 Rabbit anti RFP Invitrogen Cat# R10367 Goat Anti-Chicken IgG (H+L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A A11039, RRID: AB_142924 Bacterial and Virus Strains Erwinia carotovora ssp. carotovora 15 Basset et al., 2003 N/A Erwinia carotovora ssp. carotovora 15 pOM evf Basset et al., 2003 N/A Chemicals, Peptides, and Recombinant Proteins FD&C Blue #1 dye Spectrum Chemical FD110 Critical Commercial Assays QuantSeq 30 mRNA-Seq Library Prep Kit Lexogen SKU: 015.96 Deposited Data RNA-seq of adult and larval midguts with and without Ecc15 infection NCBI BioProject PRJNA553080 Experimental Models: Organisms/Strains Dmel/Canton-S (Cs) Bloomington RRID: BDSC_64349 Dmel/Su(H)TS (w; Su(H)-Gal4, UAS-CD8-GFP/CyO; tubGal80TS/TM6C) Zeng et al., 2010 N/A Dmel/Su(H)-lacZ (Su(H)GBE-lacZ) Furriols and Bray, 2001 N/A Dmel/Myo-lacZ (Yw; pLacW Myo-lacZ/CyO) Jiang et al., 2009 N/A Dmel/MyoTS (w; Myo1A-Gal4, UAS-GFP, Tub-Gal80TS) Jiang et al., 2009 N/A Dmel/TkvRNAi (y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS04510}attP40) Bloomington RRID: BDSC_57309 Dmel/UAS-Upd3 (w;UAS-Upd3/CyO) Buchon et al., 2009a N/A Dmel/UAS-lat;UAS-DomeDN (UAS-Latran; UAS-domeDCYT3-2) Makki et al., 2010 N/A Dmel/upd3-lacZ (Upd3.1-lacZ) Jiang et al., 2011 N/A Dmel/UAS-RasV12 (w[1118]; P{w[+mC] = UAS-Ras85D.V12}TL1) Bloomington RRID: BDSC_4847 Dmel/UAS-dpp (w[*]; P{w[+mC] = UAS-dpp.S}42B.4) Bloomington RRID: BDSC_1486 (Continued on next page) Cell Host & Microbe 26, 1–14.e1–e5, September 11, 2019 e1 ..
Article Title: A genetic circuit that extends the useful range of a BMP morphogen arose alongside insect wing evolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Phospho-Smad1/5 (Ser463/465), clone 41D10 Cell Signaling Technology Cat# 9516; RRID: AB_491015 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Mouse monoclonal anti-β-galactosidase (40-1a) Developmental Studies Hybridoma Bank Cat# 40-1a; RRID: AB_528100 Mouse monoclonal anti-Wingless (4D4) Developmental Studies Hybridoma Bank Cat# 4D4; RRID: AB_528512 Mouse monoclonal anti-Patched (Apa 1) Developmental Studies Hybridoma Bank Cat# Drosophila Ptc (Apa 1); RRID: AB_528441 mouse monoclonal anti-V5 Invitrogen Cat# 92008 (Off-The-Shelf) Guinea pig polyclonal anti-Brk Gift from Aurelio Teleman lab (DKFZ) Doumpas et al.22 N/A Chemicals, peptides, and recombinant proteins LDN-193189 dihydrochloride Tocris Cat# 6053 Trizol Invitrogen Cat# 15596026 Chloroform Sigma Aldrich Cat# C2432 Formaldehyde Thermo Scientific Cat# 28906 Hoechst 33342 Thermo Fisher Scientific Cat# H3570 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 SuperScript III kit Invitrogen Cat# 18080400 Template Switching RT Enzyme Mix NEB Cat# M0466 pMiniT vector NEB Cat# E1202 Click-iT EdU Imaging Kit Invitrogen Cat# C10338 Experimental models: Organisms/strains D. melanogaster: ubi>Tkv-HA KYOTO Stock Center DGRC: 118137 D. melanogaster: dad>lacZ or dadj1E4 BDSC 10305 D. melanogaster: UAS>dadRNAi BDSC 33759 D. melanogaster: brk>lacZ or brkXA BDSC 58792 D. melanogaster: tkv>lacZ BDSC 11191 D. melanogaster: apMI01996 BDSC 42297 D. melanogaster: GFP-Dpp Gift from Thomas Kornberg lab (UCSF) N/A D. melanogaster: UAS>tkv Gift from Michael O’Connor lab (UMN) N/A D. melanogaster: Tkv-GFP Gift from Markus Affolter lab (Basel) N/A D. melanogaster: FRT-Tkv-3xHA-FRT Gift from Markus Affolter lab (Basel) N/A D. melanogaster: dad4>EGFPnls Gift from Giorgos Pyrowolakis lab (Freiburg) N/A D. melanogaster: Omb-GFP Gift from Yohannes Bellaı̈che (Institut Curie) N/A D. melanogaster: Sal-GFP Gift from Frank Schnorrer (IBDM) N/A D. melanogaster: FRT-HA-Dpp-FRT Bosch et al.69 N/A D. melanogaster: brk[KO; mCherry] This paper N/A D. melanogaster: brk[KO; brk>Gal4] This paper N/A D. melanogaster: brk[KO; Brk-GFP] This paper N/A D. melanogaster: brk[KO; FRT-Brk-V5-FRT] This paper N/A D. melanogaster: brk[KO; BrkTd-GFP] This paper N/A (Continued on next page) ll OPEN ACCESS e1 Current Biology 35, 1–13.e1–e15, December 1, 2025 Article ..
RNA Sequencing:Article Title: Recruitment of Adult Precursor Cells Underlies Limited Repair of the Infected Larval Midgut in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-pH3 Millipore Cat# 06-570, RRID:AB_310177 rabbit anti-b-Galactosidase MP Biomedicals Cat# 0855976, RRID:AB_2334934 goat anti-b-Galactosidase MP Biomedicals Cat#0856028 mouse anti-Prospero DHSB Cat# Prospero (MR1A), RRID: AB_528440 mouse anti-Delta DSHB Cat# c594.9b, RRID:AB_528194 donkey anti-rabbit-555 Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543 donkey anti-goat-555 Thermo Fisher Scientific Cat# A-21432, RRID:AB_2535853 donkey anti-mouse-488 Thermo Fisher Scientific Cat# A-21202, RRID:AB_141607 donkey anti-mouse-647 Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542 Rabbit anti Phospho-Smad1/5 (Ser463/465) (41D10) Cell signaling Technology Cat# 9516, RRID: AB_491015 Chicken anti GFP Invitrogen Cat# A10262, RRID: AB_2534023 Rabbit anti RFP Invitrogen Cat# R10367 Goat Anti-Chicken IgG (H+L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A A11039, RRID: AB_142924 Bacterial and Virus Strains Erwinia carotovora ssp. carotovora 15 Basset et al., 2003 N/A Erwinia carotovora ssp. carotovora 15 pOM evf Basset et al., 2003 N/A Chemicals, Peptides, and Recombinant Proteins FD&C Blue #1 dye Spectrum Chemical FD110 Critical Commercial Assays QuantSeq 30 mRNA-Seq Library Prep Kit Lexogen SKU: 015.96 Deposited Data RNA-seq of adult and larval midguts with and without Ecc15 infection NCBI BioProject PRJNA553080 Experimental Models: Organisms/Strains Dmel/Canton-S (Cs) Bloomington RRID: BDSC_64349 Dmel/Su(H)TS (w; Su(H)-Gal4, UAS-CD8-GFP/CyO; tubGal80TS/TM6C) Zeng et al., 2010 N/A Dmel/Su(H)-lacZ (Su(H)GBE-lacZ) Furriols and Bray, 2001 N/A Dmel/Myo-lacZ (Yw; pLacW Myo-lacZ/CyO) Jiang et al., 2009 N/A Dmel/MyoTS (w; Myo1A-Gal4, UAS-GFP, Tub-Gal80TS) Jiang et al., 2009 N/A Dmel/TkvRNAi (y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS04510}attP40) Bloomington RRID: BDSC_57309 Dmel/UAS-Upd3 (w;UAS-Upd3/CyO) Buchon et al., 2009a N/A Dmel/UAS-lat;UAS-DomeDN (UAS-Latran; UAS-domeDCYT3-2) Makki et al., 2010 N/A Dmel/upd3-lacZ (Upd3.1-lacZ) Jiang et al., 2011 N/A Dmel/UAS-RasV12 (w[1118]; P{w[+mC] = UAS-Ras85D.V12}TL1) Bloomington RRID: BDSC_4847 Dmel/UAS-dpp (w[*]; P{w[+mC] = UAS-dpp.S}42B.4) Bloomington RRID: BDSC_1486 (Continued on next page) Cell Host & Microbe 26, 1–14.e1–e5, September 11, 2019 e1 ..
Infection:Article Title: Recruitment of Adult Precursor Cells Underlies Limited Repair of the Infected Larval Midgut in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-pH3 Millipore Cat# 06-570, RRID:AB_310177 rabbit anti-b-Galactosidase MP Biomedicals Cat# 0855976, RRID:AB_2334934 goat anti-b-Galactosidase MP Biomedicals Cat#0856028 mouse anti-Prospero DHSB Cat# Prospero (MR1A), RRID: AB_528440 mouse anti-Delta DSHB Cat# c594.9b, RRID:AB_528194 donkey anti-rabbit-555 Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543 donkey anti-goat-555 Thermo Fisher Scientific Cat# A-21432, RRID:AB_2535853 donkey anti-mouse-488 Thermo Fisher Scientific Cat# A-21202, RRID:AB_141607 donkey anti-mouse-647 Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542 Rabbit anti Phospho-Smad1/5 (Ser463/465) (41D10) Cell signaling Technology Cat# 9516, RRID: AB_491015 Chicken anti GFP Invitrogen Cat# A10262, RRID: AB_2534023 Rabbit anti RFP Invitrogen Cat# R10367 Goat Anti-Chicken IgG (H+L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A A11039, RRID: AB_142924 Bacterial and Virus Strains Erwinia carotovora ssp. carotovora 15 Basset et al., 2003 N/A Erwinia carotovora ssp. carotovora 15 pOM evf Basset et al., 2003 N/A Chemicals, Peptides, and Recombinant Proteins FD&C Blue #1 dye Spectrum Chemical FD110 Critical Commercial Assays QuantSeq 30 mRNA-Seq Library Prep Kit Lexogen SKU: 015.96 Deposited Data RNA-seq of adult and larval midguts with and without Ecc15 infection NCBI BioProject PRJNA553080 Experimental Models: Organisms/Strains Dmel/Canton-S (Cs) Bloomington RRID: BDSC_64349 Dmel/Su(H)TS (w; Su(H)-Gal4, UAS-CD8-GFP/CyO; tubGal80TS/TM6C) Zeng et al., 2010 N/A Dmel/Su(H)-lacZ (Su(H)GBE-lacZ) Furriols and Bray, 2001 N/A Dmel/Myo-lacZ (Yw; pLacW Myo-lacZ/CyO) Jiang et al., 2009 N/A Dmel/MyoTS (w; Myo1A-Gal4, UAS-GFP, Tub-Gal80TS) Jiang et al., 2009 N/A Dmel/TkvRNAi (y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS04510}attP40) Bloomington RRID: BDSC_57309 Dmel/UAS-Upd3 (w;UAS-Upd3/CyO) Buchon et al., 2009a N/A Dmel/UAS-lat;UAS-DomeDN (UAS-Latran; UAS-domeDCYT3-2) Makki et al., 2010 N/A Dmel/upd3-lacZ (Upd3.1-lacZ) Jiang et al., 2011 N/A Dmel/UAS-RasV12 (w[1118]; P{w[+mC] = UAS-Ras85D.V12}TL1) Bloomington RRID: BDSC_4847 Dmel/UAS-dpp (w[*]; P{w[+mC] = UAS-dpp.S}42B.4) Bloomington RRID: BDSC_1486 (Continued on next page) Cell Host & Microbe 26, 1–14.e1–e5, September 11, 2019 e1 ..
Plasmid Preparation:Article Title: A genetic circuit that extends the useful range of a BMP morphogen arose alongside insect wing evolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Phospho-Smad1/5 (Ser463/465), clone 41D10 Cell Signaling Technology Cat# 9516; RRID: AB_491015 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Mouse monoclonal anti-β-galactosidase (40-1a) Developmental Studies Hybridoma Bank Cat# 40-1a; RRID: AB_528100 Mouse monoclonal anti-Wingless (4D4) Developmental Studies Hybridoma Bank Cat# 4D4; RRID: AB_528512 Mouse monoclonal anti-Patched (Apa 1) Developmental Studies Hybridoma Bank Cat# Drosophila Ptc (Apa 1); RRID: AB_528441 mouse monoclonal anti-V5 Invitrogen Cat# 92008 (Off-The-Shelf) Guinea pig polyclonal anti-Brk Gift from Aurelio Teleman lab (DKFZ) Doumpas et al.22 N/A Chemicals, peptides, and recombinant proteins LDN-193189 dihydrochloride Tocris Cat# 6053 Trizol Invitrogen Cat# 15596026 Chloroform Sigma Aldrich Cat# C2432 Formaldehyde Thermo Scientific Cat# 28906 Hoechst 33342 Thermo Fisher Scientific Cat# H3570 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 SuperScript III kit Invitrogen Cat# 18080400 Template Switching RT Enzyme Mix NEB Cat# M0466 pMiniT vector NEB Cat# E1202 Click-iT EdU Imaging Kit Invitrogen Cat# C10338 Experimental models: Organisms/strains D. melanogaster: ubi>Tkv-HA KYOTO Stock Center DGRC: 118137 D. melanogaster: dad>lacZ or dadj1E4 BDSC 10305 D. melanogaster: UAS>dadRNAi BDSC 33759 D. melanogaster: brk>lacZ or brkXA BDSC 58792 D. melanogaster: tkv>lacZ BDSC 11191 D. melanogaster: apMI01996 BDSC 42297 D. melanogaster: GFP-Dpp Gift from Thomas Kornberg lab (UCSF) N/A D. melanogaster: UAS>tkv Gift from Michael O’Connor lab (UMN) N/A D. melanogaster: Tkv-GFP Gift from Markus Affolter lab (Basel) N/A D. melanogaster: FRT-Tkv-3xHA-FRT Gift from Markus Affolter lab (Basel) N/A D. melanogaster: dad4>EGFPnls Gift from Giorgos Pyrowolakis lab (Freiburg) N/A D. melanogaster: Omb-GFP Gift from Yohannes Bellaı̈che (Institut Curie) N/A D. melanogaster: Sal-GFP Gift from Frank Schnorrer (IBDM) N/A D. melanogaster: FRT-HA-Dpp-FRT Bosch et al.69 N/A D. melanogaster: brk[KO; mCherry] This paper N/A D. melanogaster: brk[KO; brk>Gal4] This paper N/A D. melanogaster: brk[KO; Brk-GFP] This paper N/A D. melanogaster: brk[KO; FRT-Brk-V5-FRT] This paper N/A D. melanogaster: brk[KO; BrkTd-GFP] This paper N/A (Continued on next page) ll OPEN ACCESS e1 Current Biology 35, 1–13.e1–e15, December 1, 2025 Article ..
Imaging:Article Title: A genetic circuit that extends the useful range of a BMP morphogen arose alongside insect wing evolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Phospho-Smad1/5 (Ser463/465), clone 41D10 Cell Signaling Technology Cat# 9516; RRID: AB_491015 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Mouse monoclonal anti-β-galactosidase (40-1a) Developmental Studies Hybridoma Bank Cat# 40-1a; RRID: AB_528100 Mouse monoclonal anti-Wingless (4D4) Developmental Studies Hybridoma Bank Cat# 4D4; RRID: AB_528512 Mouse monoclonal anti-Patched (Apa 1) Developmental Studies Hybridoma Bank Cat# Drosophila Ptc (Apa 1); RRID: AB_528441 mouse monoclonal anti-V5 Invitrogen Cat# 92008 (Off-The-Shelf) Guinea pig polyclonal anti-Brk Gift from Aurelio Teleman lab (DKFZ) Doumpas et al.22 N/A Chemicals, peptides, and recombinant proteins LDN-193189 dihydrochloride Tocris Cat# 6053 Trizol Invitrogen Cat# 15596026 Chloroform Sigma Aldrich Cat# C2432 Formaldehyde Thermo Scientific Cat# 28906 Hoechst 33342 Thermo Fisher Scientific Cat# H3570 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 SuperScript III kit Invitrogen Cat# 18080400 Template Switching RT Enzyme Mix NEB Cat# M0466 pMiniT vector NEB Cat# E1202 Click-iT EdU Imaging Kit Invitrogen Cat# C10338 Experimental models: Organisms/strains D. melanogaster: ubi>Tkv-HA KYOTO Stock Center DGRC: 118137 D. melanogaster: dad>lacZ or dadj1E4 BDSC 10305 D. melanogaster: UAS>dadRNAi BDSC 33759 D. melanogaster: brk>lacZ or brkXA BDSC 58792 D. melanogaster: tkv>lacZ BDSC 11191 D. melanogaster: apMI01996 BDSC 42297 D. melanogaster: GFP-Dpp Gift from Thomas Kornberg lab (UCSF) N/A D. melanogaster: UAS>tkv Gift from Michael O’Connor lab (UMN) N/A D. melanogaster: Tkv-GFP Gift from Markus Affolter lab (Basel) N/A D. melanogaster: FRT-Tkv-3xHA-FRT Gift from Markus Affolter lab (Basel) N/A D. melanogaster: dad4>EGFPnls Gift from Giorgos Pyrowolakis lab (Freiburg) N/A D. melanogaster: Omb-GFP Gift from Yohannes Bellaı̈che (Institut Curie) N/A D. melanogaster: Sal-GFP Gift from Frank Schnorrer (IBDM) N/A D. melanogaster: FRT-HA-Dpp-FRT Bosch et al.69 N/A D. melanogaster: brk[KO; mCherry] This paper N/A D. melanogaster: brk[KO; brk>Gal4] This paper N/A D. melanogaster: brk[KO; Brk-GFP] This paper N/A D. melanogaster: brk[KO; FRT-Brk-V5-FRT] This paper N/A D. melanogaster: brk[KO; BrkTd-GFP] This paper N/A (Continued on next page) ll OPEN ACCESS e1 Current Biology 35, 1–13.e1–e15, December 1, 2025 Article ..
|
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 